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ALFREDO JESUS IBAÑEZ GABILONDO

ALFREDO JESUS IBAÑEZ GABILONDO

ALFREDO JESUS IBAÑEZ GABILONDO


Doctor rerum naturalium (Dr. rer. nat.) (Friedrich-Schiller-Universität Jena); Licenciado en Química (PONTIFICIA UNIVERSIDAD CATOLICA DEL PERU) y Master of Science (NEW MEXICO STATE UNIVERSITY)
DOCENTE CONTRATADO - CONTRATADO
Tiempo parcial por asignaturas (TPA)
Departamento Académico de Ciencias - Sección Química

Investigaciones

Se encontraron 8 investigaciones

2017 - 2020

Developing innovative mass-spectrometry based analytical tools for rapid monitoring of wounding- and infection-related metabolites in plants

The plant defense against pathogens comprises specific cellular signaling cascades. Previously, I studied the defense response of tobacco plants against Phytophthora nicotianae by mass spectrometry (MS). However, these measurements were only snapshots in time. The objective of this proposal is to create a new MS setup that allows us to dissect the plant defense response in real-time (continuum). I will particular focus in understanding the defense mechanisms of Peruvian cultivars, such as potatoes toward local pathogens, such as Phytophthora infestans.

Participantes:

Instituciones participantes:

  • Instituto Max PlANCK de Quimica Ecologica - Departamento de Quimica BioOrganica (Financiadora)
  • max planck gesellschaft - Max Planck International - Partner Groups (Financiadora)
  • Max Planck Institute for Chemical Ecology - Department of Bioorganic Chemistry (Financiadora)
  • PONTIFICIA UNIVERSIDAD CATOLICA DEL PERU - Instituto de Ciencias Omicas y Biotecnologia Aplicada (Financiadora)
2017 - 2019

Análisis retrospectivo de los precursores de aroma y componentes de sabor del cacao fino de aroma

El presente proyecto tiene como objetivo el expandir el actual conocimiento sobre las moléculas orgánicas de bajo peso molecular, MOBPM, que contribuyen al sabor de los productos obtenidos a partir del cacao para luego aplicarlo en el proceso industrial de una entidad asociada. El aspecto innovador consiste en el uso de espectrómetros de masas y nuestra estrategia de análisis denominada análisis retrospectivo. Es decir, evaluaremos cómo los precursores de sabor, entendiéndose ¿sabor¿ como la suma de componentes de aroma, gustativos y táctiles, son transformados en componentes de sabor durante los procesos de fabricación del chocolate. La muestra a estudiar será el cacao porcelana de Piura. No obstante, los resultados obtenidos podrán ser extendidos a cacaos finos de otras zonas del país. El presente trabajo de investigación tiene tres objetivos: (1) Completar, utilizando análisis propio y data ya existente, una base de datos de los MOBPM responsables del sabor y la buena calidad de un chocolate producido a partir del cacao fino de aroma. (2) Seguir todo el proceso de desarrollo del sabor, es decir identificar reacciones químicas y bioquímicas indicativas de cómo los precursores de sabor se transforman en compuestos de sabor (identificados en Objetivo 1). Esta identificación se llevará acabo siguiendo una filosofía de retro síntesis (desde el chocolate hasta el cacao). (3) Aplicar los resultados para definir parámetros de calidad del procesamiento industrial del cacao fino.

Participantes:

Instituciones participantes:

  • PONTIFICIA UNIVERSIDAD CATOLICA DEL PERU - ICOBA PUCP (Financiadora)
  • PONTIFICIA UNIVERSIDAD CATOLICA DEL PERU - Vicerrectorado de investigación (Financiadora)
  • Programa Nacional de Innovación Agraria - Jefe de la Unidad Descentralizada 3 (Financiadora)
  • Theobroma S.A.C. - Theobroma S.A.C. (Financiadora)
2013 - 2016

Ambizione: Single-cell (S.cerevisiae) level examination of the telomere maintenance process

Aging affects a countless number of chemical and biological processes within the cell. Put simply, aging can be pictured as a shift from fully functional to failing-to-function. Although cell aging is not biologically programmed, the cell viability is. Cell viability is controlled via cell regulation and cell maintenance mechanisms. Interestingly, some of the components of these two cell mechanisms are inherently stochastic. These stochastic characteristics can then propagate through the biological network system, giving rise to the more complex observable traits that are associated with aging. The goal of this proposal is to understand how the S. cerevisiae metabolic network maintains homeostasis using a novel analytical platform for monitoring metabolic network changes as a consequence of gene deletion in order to induce cellular telomerase dysfunction, and trigger the alternative lengthening of telomeres (ALT) phenotype in cells.

Participantes:

Instituciones participantes:

  • EMBL-EBI - Stegle research group (Financiadora)
  • ETH Zurich - DEPARTMENT of Chemistry and Applied biosciences (Financiadora)
  • ETH Zurich - Institute of Biochemistry (Financiadora)
  • ETH Zurich - Institute of Molecular Systems Biology (Financiadora)
  • Swiss National Science Foundation - Ambizione (Financiadora)
2011 - 2013

Marie Curie Intra-European Fellowship (FP7): DiSC-MS - Direct Single cell Mass Spectrometry: a novel analytical platform for monitoring the metabolism at single-cell level

Cells can display different variations in behavior toward different external cues. The multidisciplinary field of metabolomics provides a unique insight into the molecular feedbacks and cross-talk between metabolic cascades that allow organisms to adapt toward different sources of biotic and abiotic stress. Unfortunately, there is still a substantial technological limitation (in terms of sensitivity and throughput), when trying to understand the complex and highly interrelated metabolic processes occurring within a clonal cell population under stress, and how these metabolic processes could lead to the generation of resistant cells toward a specific stress cue (i.e. stochastically-induced phenotypic heterogeneity). The objective of this proposal is to overcome the substantial technological limitation in single cell metabolomic analysis by developing an automated microfluidic device couple to a mass spectrometer for obtaining high quality ¿metabolic profiles¿; in order to build models of signal cascades and of metabolic activity occurring in single cell organisms under different nutrient stress conditions. The proposed single cell metabolomic instrumentation belong to a group of analytical platforms, we called Direct Single Cell - Mass Spectrometry (DiSC-MS) platforms, currently the forerunners of single cell metabolomic studies are in Japan and in the USA, while only a handful of groups in Europe are also active in this field. Although the proposal focuses on monitoring the metabolic profiles of E. coli and S. cerevisiae to identify resistant phenotypes within a large clonal population toward a nutritional shift, the developed analytical method may also be used to better understand, how some populations of bacteria can exploit their regulatory metabolic feedback to create multiple types of coexisting phenotypes that are drug resistant.

Participantes:

Instituciones participantes:

  • ETH Zurich - DEPARTMENT of Chemistry and applied biosciences (Financiadora)
  • rijksuniversiteit groningen - Molecular Systems Biology (Financiadora)
  • Union Europea - Marie Curie Intra-European Fellowship (FP7) (Financiadora)
2008 - 2010

Alexander von Humboldt: Development of UV-MALDI- & IR-LDI-IMS methodology for discovery of lipid-based biomarkers for cross-kingdom pathogens

Inflammation is one of the multiple complex biological responses against harmful stimuli such as pathogens, and damaged cells. Abnormalities associated with inflammation comprise a large group of disorders which are related to a variety of diseases. One of the biological pathways activated during the inflammation response is the Lipoxygenase pathway, which is common in plant as well as in animal tissue. The goal of this research project is the development of a Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) methodology for discovery of lipid-based biomarkers produced during a pathogen attack on healthy plant and animal tissue.

Participantes:

Instituciones participantes:

  • FUNDACION ALEXANDER VON HUMBOLDT, ALEMANIA - Post-doctoral fellowship (Financiadora)
  • Max planck Institute for Chemical Ecology - Department of Molecular Ecology (Financiadora)
  • Universitaetsklinikum Muenster - Institute of Medical Physics and Biophysics (Financiadora)
  • Westfälische Wilhelms-Universität Münster - Institute of Botany (Financiadora)
2005 - 2008

International Max Planck Research School fellowship - Lab-on-a-chip platform for proteomics studies

The ability to perform proteomics analysis quickly and easily is becoming increasingly important in the fields of bioanalytical chemistry. The success of the high-throughput proteomic technique know as matrix-assisted laser/desorption ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) is related to the sample preparation prior to the analysis.The goal of this project is to overcome the deficiencies of the state-of-the-art methods for proteins sample preparation, such as Zip-Tips(r) and dialysis-based methods. To accomplish our goal, we introduce a proteomic tool known as plastic-MALDI substrates (pMALDI). The pMALDI chips can address the deficiencies of the state-of-the-art methods and out-perform them in a variety of genomics and proteomics applications.

Participantes:

Instituciones participantes:

  • max planck gesellschaft - International max planck research school (Financiadora)
  • Max Planck Institute for Chemical Ecology - Mass spectrometry and proteomic research group (Financiadora)
1999 - 2000

DESARROLLO DE UN INMUNOSENSOR PARA LA DETECCION DE T3

Participantes:

Instituciones participantes:

  • UNIVERSIDAD CATOLICA DEL PERU, FACULTAD DE QUIMICA (Financiadora)
1998 - 1999

ESPECTROSCOPIA SPECKEL PARA LA MEDICION DE CORROSION

Participantes:

Instituciones participantes:

  • UNIVERSIDAD CATOLICA DEL PERU (Financiadora)