Alexander von Humboldt: Development of UV-MALDI- & IR-LDI-IMS methodology for discovery of lipid-based biomarkers for cross-kingdom pathogens

Inflammation is one of the multiple complex biological responses against harmful stimuli such as pathogens, and damaged cells. Abnormalities associated with inflammation comprise a large group of disorders which are related to a variety of diseases. One of the biological pathways activated during the inflammation response is the Lipoxygenase pathway, which is common in plant as well as in animal tissue. The goal of this research project is the development of a Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) methodology for discovery of lipid-based biomarkers produced during a pathogen attack on healthy plant and animal tissue.

International Max Planck Research School fellowship - Lab-on-a-chip platform for proteomics studies

The ability to perform proteomics analysis quickly and easily is becoming increasingly important in the fields of bioanalytical chemistry. The success of the high-throughput proteomic technique know as matrix-assisted laser/desorption ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) is related to the sample preparation prior to the analysis.The goal of this project is to overcome the deficiencies of the state-of-the-art methods for proteins sample preparation, such as Zip-Tips(r) and dialysis-based methods. To accomplish our goal, we introduce a proteomic tool known as plastic-MALDI substrates (pMALDI). The pMALDI chips can address the deficiencies of the state-of-the-art methods and out-perform them in a variety of genomics and proteomics applications.

DESARROLLO DE UN INMUNOSENSOR PARA LA DETECCION DE T3

ESPECTROSCOPIA SPECKEL PARA LA MEDICION DE CORROSION